Question 2 : Restriction map for a specific RE
Tushar Choudhary - 2019111019
Information regarding the RE and genomic sequence used
I have tested my code against many test cases involving different REs and genomic sequences.
The following report covers test details where the genomic used is
pBR322, the RE is
pBR322 is one of the most commonly used E.coli cloning vectors.
When tested with
NaeI enzyme is gives 4 restriction sites of
GCCGGC and hence the sequence is broken into 5 fragments.
Output for my code
Output for mapper
Link to the mapper used : http://www.restrictionmapper.org/
As mentioned before, the enzyme finds 4 matches for the restriction site and the sequence is hence broken into 5 fragements. Both the output from the mapper and my code give the same position for the matches found found (displacement of 2 due to indices being shifted by two).
Hence the outputs from my code and mapper match.