Retrieve the nucleotide from FASTA accession number.

Authors: Thomsen, L, Soikum, S, and Soikum, K and Makovetskyi, S.M.

Below you have 3 options. Either chose a "FASTA_example" gene, or enter a "FASTA_accession_number" or paste and unspecified DNA sequence in the text field. Your selection will be used as input sequence to the Primer3 design algorithm.

If you leave your email below we will send you a nicely formatted HTML report of the results. Each time you run the program you get a new report. We dont store your email here, but a copy is sent to our support email. The program also runs without entering an email

Or you can chose to enter the FASTA accession number directly and the simulator will download the sequence

Or you can chose to paste an unspecified DNA sequence

Now we design primers based on the sequence

Here we plot the primers position along the target sequence

Now we run the realtime PCR and melting curve analysis

Salt, primer and MgCl2 concentrations can be chosen from the fixed values below

Here is the debug output from the PCR and melting analysis algorithms. The PCR cycles are tagged by time stamps showing how fast thie amplication can be done by the RAPDNA - integrated sample prep and qPCR machine

And finally we run a capillary electrophoresis on the amplified products